Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,457 results for: golden gate

Clear keywords
Showing: 2561 - 2580 of 5457 results

  2. pMVRha

    Plasmid
    #229553
    Purpose
    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
    Depositor
    Sandy Schmidt
    Publication
    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229553-plasmid-map-sequence-id-461178

    Enlarge

  3. pHW1385

    Plasmid
    #229879
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Publication
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    Tags
    mScarlet-I-C1
    Expression
    Worm
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229879-plasmid-map-sequence-id-460859

    Enlarge

  5. PAC_E83_LOV_eGFP

    Plasmid
    #231574
    Purpose
    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light
    Depositor
    Dominik Niopek
    Publication
    Wolf et al Nat Methods. 2025 Aug;22(8):1698-1706. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    Expression
    Mammalian
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    231574-plasmid-map-sequence-id-460758

    Enlarge

  7. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227892
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227892-plasmid-map-sequence-id-455731

    Enlarge

  9. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227899
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227899-plasmid-map-sequence-id-452509

    Enlarge

  10. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227902
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227902-plasmid-map-sequence-id-453977

    Enlarge

  11. pKSEe401R

    Plasmid
    #218532
    Purpose
    CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.
    Depositor
    Kirill Demchenko
    Publication
    Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    218532-plasmid-map-sequence-id-458122

    Enlarge

  12. pM-mTagBFP2

    Plasmid
    #228615
    Purpose
    Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction
    Depositor
    Marc Birtwistle
    Publication
    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTagBFP2
    Expression
    Mammalian
    Mutation
    We introduced some silent mutations to avoid esse…
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228615-plasmid-map-sequence-id-457660

    Enlarge

Showing: 2561 - 2580 of 5457 results