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pFPO-His-mTurquoise2_T203Y

Plasmid

#191456

Purpose

Bacterial isolation of mTurquoise2 with a T203Y mutation

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mTurquoise2_T203Y

Use

Tags

His

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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25Q-ymsfGFP

Plasmid

#128559

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymsfGFP

Use

Tags

ymsfGFP

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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68Q-ymScarlet

Plasmid

#128562

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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25Q-ymScarlet

Plasmid

#128561

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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4xmts-G-Ca-FLITS

Plasmid

#191462

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

4xmts

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Insert

(Cyto)iGlucoSnFR

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ER-G-Ca-FLITS-KDEL

Plasmid

#191464

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

ER and KDEL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SRP19 in pLX311

Plasmid

#233292

Purpose

Expression of WT SRP19

Depositor

Sefi Rosenbluh

Insert

SRP19 (SRP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-EGFP

Plasmid

#191458

Purpose

Compare the intensity of EGFP to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

EGFP

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

Plasmid

#191459

Purpose

Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mTurquoise2_T203Y

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mCherry

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFPO-His-G-Ca-FLITS

Plasmid

#191455

Purpose

Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

His

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

Plasmid

#164510

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Has Service

AAV9

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

Synapsin

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

jRCaMP1b

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PC2328

Plasmid

#232089

Purpose

Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.

Depositor

Pranam Chatterjee

Article

Hong et al Nat Commun 16, 3555 (2025). https://doi.

Insert

duAb with cloning Esp3I restriction site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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SRP19 (siRNA resistant) in pLX311

Plasmid

#233291

Purpose

Expression of WT SRP19 (siRNA resistant)

Depositor

Sefi Rosenbluh

Insert

SRP19 (SRP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Expresses WT SRP19 that is resistant to siRNA

Promoter

E1Fa

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

RCaMP1h

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

Plasmid

#191457

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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