Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,130 results for: Tef

Showing: 241 - 260 of 1130 results

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p416TEF1 Su9-Cystector (Cysteine biosensor)

Plasmid

#250655

Purpose

Mitochondrial matrix expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiae

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Su9-Cystector (Cysteine biosensor)

Tags

Mitochondrial targetting sequence based on Neuros…

Expression

Yeast

Promoter

TEF1

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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p416TEF1 Cystector (Cysteine biosensor)

Plasmid

#250654

Purpose

Cytosolic expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiae

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Yeast

Promoter

TEF1

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC61-FAPoptim

Plasmid

#221126

Purpose

FAP-tagged marker for the ER (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-SEC63-FAPoptim

Plasmid

#221116

Purpose

FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim-SEC63

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-SEC61-FAPoptim

Plasmid

#221115

Purpose

FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-STE3-GFP

Plasmid

#221113

Purpose

GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

STE3-GFP

Expression

Yeast

Promoter

TEF1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ANP1-FAPoptim

Plasmid

#221120

Purpose

FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ANP1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ERG6-FAPoptim

Plasmid

#221121

Purpose

FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ERG6-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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YIplac128-TEF2p-LDH-CYC1t

Plasmid

#216120

Purpose

Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine)

Expression

Yeast

Promoter

TEF2 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

Plasmid

#139963

Purpose

Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

Plasmid

#139964

Purpose

Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 241 - 260 of 1130 results

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