Addgene

We narrowed to 16,701 results for: URE

Showing: 241 - 260 of 16701 results

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Pediculus humanus corporis PINK1 (143 - 575)

Plasmid

#110751

Purpose

Bacterial expression of PhPINK1 (crystallography)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Use

Tags

N-His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCX4-EGFP

Plasmid

#36120

Depositor

Larry Goldstein

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

eGFP (eGFP )

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pMB38-HF380

Plasmid

#37332

Depositor

Takahide Kon

Article

Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

Insert

Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)

Use

Dictyostelium expression

Tags

His6 and FLAG tag

Expression

Mutation

deleted amino acids 1-1387

Promoter

TRE-Pmin

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
mEos2-Talin-IBS2-7

Plasmid

#57424

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin IBS2 (Tln1 Mouse)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-Vinculin-14

Plasmid

#57691

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Vinculin (VCL Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-ABM-10

Plasmid

#57421

Purpose

Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin AMB

Use

Tags

mEos2

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p3A2-HA

Plasmid

#234804

Purpose

Mammalian expression of bovine arrestin-2 with C-terminal HA tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-2 (ARRB2 Bovine)

Use

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-DR

Plasmid

#234800

Purpose

Mammalian expression of bovine arrestin-3 (D291R)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

D291R

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3A3-HA

Plasmid

#234806

Purpose

Mammalian expression of bovine arrestin-3 with C-terminal HA tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3A2-Flag

Plasmid

#234803

Purpose

Mammalian expression of bovine arrestin-2 with C-terminal Flag tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-2 (ARRB2 Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3VEA3

Plasmid

#234796

Purpose

Mammalian expression of bovine arrestin-3

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-Tr

Plasmid

#234798

Purpose

Mammalian expression of truncated bovine arrestin-3 (1-393)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

truncated (1-393)

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-RE

Plasmid

#234799

Purpose

Mammalian expression of bovine arrestin-3 (R170E)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

R170E

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KQ

Plasmid

#64982

Purpose

Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108Q

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB38-HF380deltaMTBD

Plasmid

#37333

Depositor

Takahide Kon

Article

Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

Insert

Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)

Use

Dictyostelium expression

Tags

His6 and FLAG tag

Expression

Mutation

deleted amino acids 1-1387 and replaced amino aci…

Promoter

TRE-Pmin

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only
MT363

Plasmid

#65858

Purpose

The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

nahG

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE3

Plasmid

#108380

Purpose

Mammalian expression vector for xCas9(3.7)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE3

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE4

Plasmid

#108381

Purpose

Mammalian expression vector for xCas9(3.7)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE4

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge