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Plasmid#162908PurposeGateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-JNKKTR -miRFP670-WPRE
Plasmid#159976Purposesingle wavelength translocating reporter of the JNK pathwayDepositorInsertJNKKTR -miRFP670
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(F178A/D474N)
Plasmid#119694PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationF178A/D474NPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-NDP52(V136S/C443K)
Plasmid#119696PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant NDP52DepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationV136S/C443KPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3
Plasmid#200635PurposeNegative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cellsDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo
Plasmid#62345PurposeExpresses MCP-tdiRFP670 in mammalian cellsDepositorInsertMCP-tdiRFP670
UseTagsHA and SV40 NLSExpressionMammalianMutationPromoterCAG promoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-miRFP670nano-PGK-Blast
Plasmid#196722PurposeTet-inducible expression of Cas9-T2A-miRFP670nanoDepositorInsertCas9-T2A-miRFP670nano
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP
Plasmid#197370PurposeThe lentiviral vector for miRFP670nano(R57C/C86S) and EGFPDepositorInsertmiRFP670nano(R57C/C86S)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationR57C/C86S mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190078Purposeexpress Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (TMPRSS2 Rhinolophus ferrumequinum)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro
Plasmid#166133PurposeThis plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting cldn1 gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-H2b-emiRFP670-P2A-TEVP
Plasmid#174379PurposeAAV virus for expressing TEVP and H2b-emiRFP670DepositorInsertTEVP
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E34: attB5r-iRFP670-nostop-attB1r
Plasmid#162947PurposeGateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin
Plasmid#223962PurposeDual fluorescent reporter for histone H2B and for exogenous RbDepositorUseTagsYFP and iRFP670ExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670
Plasmid#169591PurposeTier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-2Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-336 N-Tag miRFP670[2] L32 {Esp3I(2x)} EGFP
Plasmid#129839PurposePPI (BiFC/TriFC) assay vectorDepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-209 C-Tag miRFP670[2] L10 FRB EGFP
Plasmid#129894PurposePPI (BiFC/TriFC) assay vectorDepositorInsertFRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR
Plasmid#190079Purposeexpress chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (TMPRSS2 Rhinolophus alcyone and Rhinolophus ferrumequinum, Synthetic)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670
Plasmid#169592PurposeTier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).DepositorInsertphloretin-controlled SEAP and iRFP expression
UseTagsExpressionMammalianMutationPromoterTtgO2-PCMVmin-2Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only