We narrowed to 558 results for: nif
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Plasmid#115506PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celBDepositorInsertRlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt7B-V5
Plasmid#35934DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianPromoterEF-1-alpha promoterAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Wnt10A STOP
Plasmid#35883DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt4 STOP
Plasmid#35873DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMEL15
Plasmid#107921Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 full-length (1–510)
Plasmid#193858PurposeExpression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
Tags3C protease cleavage site and 6x-His tagExpressionBacterialMutationCodon optimised for expression in E. coliPromoterT7Available SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FLJ23445
Plasmid#15429DepositorAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKOdeltagalK (Kan)
Plasmid#110089PurposeAnalogous to pKO but without the negative selection gene galK.DepositorTypeEmpty backboneUseBacterial knockout generationAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKOdeltagalK (Hyg)
Plasmid#110090PurposeAnalogous to pKO but without the negative selection gene galK.DepositorTypeEmpty backboneUseBacterial knockout generationAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Apr)
Plasmid#110083PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Let7-SunTag
Plasmid#196928PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-TREAT
Plasmid#196921PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporterDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-SunTag
Plasmid#196929PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR21mutant-IRE-TREAT
Plasmid#196931PurposeMutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporterDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR21-IRE-TREAT
Plasmid#196930Purposemir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-IRE-TREAT
Plasmid#196934PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7-IRE-TREAT
Plasmid#196925PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only