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GreenT-EC.b - Display - CyRFP1

Plasmid

#193944

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.c - Display - CyRFP1

Plasmid

#193945

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - pRSET

Plasmid

#193947

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Tags

His-Tag

Expression

Bacterial

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - GPI

Plasmid

#193946

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Tags

GPI-anchor and Igk_secretion - HA-Flag

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMA3200

Plasmid

#46855

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Promoter

TRE-Tight

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.b - pRSET

Plasmid

#193948

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

His-Tag

Expression

Bacterial

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GreenT-EC.c - pRSET

Plasmid

#193949

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Tags

His-Tag

Expression

Bacterial

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3228

Plasmid

#46856

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY K81M

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Promoter

TRE-Tight

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3383

Plasmid

#46875

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)

Use

Retroviral

Promoter

LTR

Available Since

Aug. 13, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Human RNF149-3X FLAG

Plasmid

#225332

Purpose

Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Human RNF130-3X FLAG

Plasmid

#225331

Purpose

Lentiviral expression of human RNF130 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 (RNF130 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3379

Plasmid

#46874

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Promoter

TRE-Tight

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X MYC

Plasmid

#225333

Purpose

Lentiviral expression of human RNF149 with 3x myc tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xMyc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Luciferase shRNA

Plasmid

#225343

Purpose

shRNAmir backbone for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

Luciferase shRNA (LOC116160065 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMA3431

Plasmid

#46876

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Mutation

R592Q

Promoter

TRE-Tight

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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RNF130 shRNA

Plasmid

#225340

Purpose

shRNAmir backbone for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3411

Plasmid

#46877

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase1 beta 3 (Gucy1b3 Rat)

Use

Retroviral

Mutation

E473K, C541D

Promoter

LTR

Available Since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
RNF130 shRNA-TRE

Plasmid

#225336

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-mMAPT

Plasmid

#231584

Purpose

Minigene of mouse MAPT containing exon 9, 10, 11 and relevant intronic sequences

Depositor

Chaolin Zhang

Article

Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

Insert

mouse MAPT (Mapt Mouse)

Expression

Mammalian

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge