We narrowed to 5,179 results for: pcDNA3.1+

1. SPLICS Po-Mt Short P2A

   Plasmid

   #164116

   Purpose

   Detect the short Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS Po-Mt Long P2A

   Plasmid

   #164115

   Purpose

   Detect the long Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-FlipCherry-T2A-GFP

   Plasmid

   #124436

   Purpose

   Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipCherry-T2A-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HDAC5 Flag

   Plasmid

   #13822

   Purpose

   Mammalian expression of human histone deacetylase 5 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-SREBP-2

   Plasmid

   #204200

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Use

   Tags

   T7x3

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HDAC8 Flag

   Plasmid

   #13825

   Depositor

   Eric Verdin

   Article

   Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

   Insert

   HDAC8 (HDAC8 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HDAC7 Flag

   Plasmid

   #13824

   Purpose

   Mammalian expression of human histone deacetylase 7 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC7 (HDAC7 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA-FLAG-METTL3

   Plasmid

   #160250

   Purpose

   Express FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

11. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-NLS-AcrIIA4

    Plasmid

    #113037

    Purpose

    Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SV40 NLS-AcrIIA4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GA-NES

    Plasmid

    #61018

    Purpose

    ddGFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. nLuc-PTHR1

    Plasmid

    #162577

    Purpose

    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    Parathyroid Hormone Receptor 1 (PTH1R Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 HA-Sumo1 WT

    Plasmid

    #48966

    Purpose

    Expresses HA-Sumo1 WT in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMYC-GFP

    Plasmid

    #42142

    Depositor

    Olufunmilayo Olopade

    Article

    Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

    Insert

    c-Myc (MYC Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTE4398

    Plasmid

    #74042

    Purpose

    Expresses human codon-optimized LbCpf1 and Lb crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Lb crRNA
    LbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. HiBiT-FZD1

    Plasmid

    #195839

    Purpose

    Expresses human FZD1 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD1 (FZD1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRGEN-CMV-CjCas9

    Plasmid

    #89752

    Purpose

    Expression of CjCas9 in mammalian cells

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Green Pegassos

    Plasmid

    #163114

    Purpose

    Expresses biosensors for pyruvate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Pegassos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Klotho (membrane)

    Plasmid

    #17712

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    P101S

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. Green Lindoblum

    Plasmid

    #163113

    Purpose

    Expresses biosensor for lactate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Lindolum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-f:PGC1

    Plasmid

    #1026

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-HCMVgL-FLAG (TB40/E)

    Plasmid

    #136449

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Use

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mKO3-TEAD4

    Plasmid

    #221217

    Purpose

    RFP fused TEAD4

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    mKO3-TEAD4 (TEAD4 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SPLICS PM-ER Long P2A

    Plasmid

    #164111

    Purpose

    Detect the long Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-I-PhoCl-HCVp

    Plasmid

    #87697

    Purpose

    Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
    

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 HA Arf1 ActQ71L

    Plasmid

    #10832

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf1 Q71L (ARF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q71L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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