We narrowed to 2,324 results for: PUC19

1. qTAG-N-Blast-moxGFP

   Plasmid

   #207677

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. qTAG-C-V5-Zeo

   Plasmid

   #207741

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDD283

   Plasmid

   #66824

   Purpose

   YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   YPET-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized YPET

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2SK

   Plasmid

   #59383

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-kan
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-miRFP670nano3-Blast

   Plasmid

   #207713

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-N-Zeo-mScarlet

   Plasmid

   #207700

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pLoxP-DHFR-mCherry

   Plasmid

   #70147

   Purpose

   Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

   Depositor

   David Sibley

   Article

   Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

   Insert

   a selectable dihydrofolate reductase-thymidylate synthase marker
   

   Use

   Expression in toxoplasma gondii

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   DHFR 5' UTR

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Blast-mNeon

   Plasmid

   #207676

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD317 (Pmyo-2::GFP + SEC)

    Plasmid

    #73344

    Purpose

    SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

    Insert

    Pmyo-2::GFP + SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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