We narrowed to 3,005 results for: EED

1. pHEE-R-kan9si

   Plasmid

   #222833

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHEE-R-hyg9si

   Plasmid

   #222832

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHEE-R-hyg5A9si

   Plasmid

   #222835

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHEE-R-bar5A9si

   Plasmid

   #222837

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, bialaphos and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHEE-R-bar9si

   Plasmid

   #222834

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOpen-ECOligA

   Plasmid

   #165564

   Purpose

   Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   E. coli DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pFGL821

   Plasmid

   #58223

   Purpose

   For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

   Depositor

   Naweed Naqvi

   Article

   Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

   Insert

   hygromycin B phosphotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFGL821-8

   Plasmid

   #58224

   Purpose

   For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

   Depositor

   Naweed Naqvi

   Article

   Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

   Insert

   hygromycin B phosphotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFGL822

   Plasmid

   #58225

   Purpose

   For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

   Depositor

   Naweed Naqvi

   Article

   Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

   Insert

   phosphinothricin acetyl transferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFGL822-8

    Plasmid

    #58226

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    phosphinothricin acetyl transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFRm43GW

    Plasmid

    #133748

    Purpose

    Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana

    Depositor

    Niko Geldner

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTD103aiiA

    Plasmid

    #48884

    Purpose

    Negative feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

    Insert

    aiiA and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA (TSAANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFG7m34GW

    Plasmid

    #133747

    Purpose

    Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

    Depositor

    Niko Geldner

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFGL820

    Plasmid

    #58221

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    ILV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ILV2 native promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFGL820-2

    Plasmid

    #58222

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    ILV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ILV2 native promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-6xHis-GFP(-7)-neg_tag1

    Plasmid

    #219419

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    GFP(-7)-neg_tag1
    

    Use

    Tags

    DEEEDD and MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDSG546

    Plasmid

    #214212

    Purpose

    Arabinose-inducible folA (used in cross-feeding experiment)

    Depositor

    Uri Alon

    Article

    Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

    Insert

    AraC, folA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDSG550

    Plasmid

    #214213

    Purpose

    Arabinose-inducible trpC (used in cross-feeding experiment)

    Depositor

    Uri Alon

    Article

    Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

    Insert

    AraC, trpC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW004

    Plasmid

    #131805

    Purpose

    Vector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    Zea mays FNR and Z. mays SIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNJB80

    Plasmid

    #51518

    Purpose

    Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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