We narrowed to 34,848 results for: CaS;

1. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA47_dCas9-GFP_NOT_i1

   Plasmid

   #231416

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. lvl0-G-CasE

   Plasmid

   #229184

   Purpose

   lvl 0 5 gene

   Depositor

   Ron Weiss

   Article

   Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

   Insert

   CasE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. lvl0-5-CaseR

   Plasmid

   #229169

   Purpose

   lvl 0 5 prime UTR

   Depositor

   Ron Weiss

   Article

   Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

   Insert

   CasE restiction site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pdnCas3-CDA1

   Plasmid

   #229535

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   dnCas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdnCas3-APOBEC1

   Plasmid

   #229537

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   dnCas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas3-APOBEC1

   Plasmid

   #229536

   Purpose

   pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   Cas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas3-CDA1

   Plasmid

   #229534

   Purpose

   pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   Cas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

    Plasmid

    #222988

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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