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We narrowed to 7,122 results for: ef1a

Showing: 6551 - 6600 of 7122 results

  1. ARB287b

    Plasmid
    #124016
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-SV40pA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124016-plasmid-map-sequence-id-252439

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  2. ARB287c

    Plasmid
    #124017
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-rglpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124017-plasmid-map-sequence-id-252443

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  8. LLP617_αGCN4-mCherry

    Plasmid
    #211785
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-DNMT3A
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211785-plasmid-map-sequence-id-417372

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  10. LLP618_αGCN4-KRAB-CO

    Plasmid
    #211782
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-mCherry
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect mCherr…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211782-plasmid-map-sequence-id-417156

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  11. pTM

    Plasmid
    #162783
    Purpose
    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Type
    Empty backbone
    Use
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Mutation
    Promoter
    CHO Elongation factor 1A
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162783-plasmid-map-sequence-id-333802

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  16. LLP779_αGCN4-VP64-CO

    Plasmid
    #211772
    Purpose
    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-VP64
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect VP64 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211772-plasmid-map-sequence-id-417389

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  17. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211784-plasmid-map-sequence-id-417361

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  21. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  22. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

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  23. pDiCre-mCherry

    Plasmid
    #164572
    Purpose
    Integration of a DiCre cassette in PbGFP parasite genome
    Depositor
    Olivier Silvie
    Article
    Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d
    Inserts
    FKBP12-CRE59
    FRB-CRE60
    mCherry
    Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164572-plasmid-map-sequence-id-325185

    Enlarge

  25. TFORF2158

    Plasmid
    #141949
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BCL11B (BCL11B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    141949-plasmid-map-sequence-id-395867

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  26. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128386-plasmid-map-sequence-id-263327

    Enlarge

  27. TFORF0117

    Plasmid
    #141524
    Purpose
    Lentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141524-plasmid-map-sequence-id-276708

    Enlarge

  30. TFORF1686

    Plasmid
    #143966
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF1B (HNF1B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143966-plasmid-map-sequence-id-404622

    Enlarge

  31. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

    Enlarge

  32. HR_G3BP1-V5-APEX2-GFP

    Plasmid
    #105284
    Purpose
    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
    Use
    Human homologous recombination donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105284-plasmid-map-sequence-id-229022

    Enlarge

  33. TFORF0118

    Plasmid
    #141525
    Purpose
    Lentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141525-plasmid-map-sequence-id-276711

    Enlarge

  34. TFORF1355

    Plasmid
    #143100
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ILF3 (ILF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143100-plasmid-map-sequence-id-399020

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  36. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid
    #122064
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1a（1.1kb short version)
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122064-plasmid-map-sequence-id-242270

    Enlarge

  37. TFORF0612

    Plasmid
    #143054
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBPJ (RBPJ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143054-plasmid-map-sequence-id-399034

    Enlarge

  39. TFORF3055

    Plasmid
    #144531
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    THRB (THRB Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    144531-plasmid-map-sequence-id-471167

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  40. TFORF3056

    Plasmid
    #144532
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    THRB (THRB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144532-plasmid-map-sequence-id-404212

    Enlarge

  41. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

    Enlarge

  43. TFORF3253

    Plasmid
    #144729
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBPJ (RBPJ Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144729-plasmid-map-sequence-id-404330

    Enlarge

  44. TFORF0257

    Plasmid
    #141560
    Purpose
    Lentiviral vector for overexpressing the TCF4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF4 (TCF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141560-plasmid-map-sequence-id-276816

    Enlarge

  45. TFORF0452

    Plasmid
    #143879
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    THRA (THRA Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143879-plasmid-map-sequence-id-404555

    Enlarge

  46. TFORF2159

    Plasmid
    #143347
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BCL11B (BCL11B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143347-plasmid-map-sequence-id-400332

    Enlarge

  47. TFORF1687

    Plasmid
    #143836
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF1B (HNF1B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143836-plasmid-map-sequence-id-391576

    Enlarge

  49. TFORF0267

    Plasmid
    #142502
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF4 (TCF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142502-plasmid-map-sequence-id-397249

    Enlarge

Showing: 6551 - 6600 of 7122 results