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We narrowed to 23,602 results for: CRISPR

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Showing: 2621 - 2640 of 23602 results

  1. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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  2. pDestTol2CG2-U6:gRNA

    Plasmid
    #63156
    Purpose
    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63156-plasmid-map-sequence-id-177022

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  6. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  10. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Expression
    Yeast
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  12. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-472945

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  20. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    N/A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102563-plasmid-map-sequence-id-193893

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Showing: 2621 - 2640 of 23602 results