We narrowed to 7,096 results for: cas9 plasmid

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJA14

Plasmid

#59929

Purpose

gRNA for cleavage at rde-1(H974) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(H974) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ230-TREX2-N

Plasmid

#244025

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

TREX2

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ230-AtEXO1B-N

Plasmid

#244024

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

AtEXO1B

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCB-CRISPRi

Plasmid

#221136

Purpose

Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

cbu1169 promoter

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG2

Plasmid

#90277

Purpose

(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg2)

Use

A. niger

Expression

Bacterial

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJA46

Plasmid

#59928

Purpose

gRNA for cleavage at rde-1(D801) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D801) gRNA

Use

CRISPR

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJA45

Plasmid

#59927

Purpose

gRNA for cleavage at rde-1(D718) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D718) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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PEA1-GFP

Plasmid

#171993

Purpose

Delivers all prime editing nickase components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PEA1-Puro

Plasmid

#171991

Purpose

Delivers all prime editing nickase components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMEL13

Plasmid

#107919

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL12

Plasmid

#107918

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

Plasmid

#169921

Purpose

DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

IRES-Puro-pEF1a-EGFP

Use

CRISPR

Promoter

EF1a

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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