We narrowed to 3,092 results for: pcdna3 1

1. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GLG1-2Strep

   Plasmid

   #210362

   Purpose

   Expresses GLG1 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   GLG1 (GLG1 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NSP6-FR-2Strep

   Plasmid

   #210317

   Purpose

   Expresses SARS-CoV-2 NSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS0035

   Plasmid

   #131125

   Purpose

   Mammalian Target-AID expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPK-351

   Plasmid

   #157921

   Purpose

   pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

   Depositor

   Phillip Kyriakakis

   Article

   Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

   Inserts

   PIF3-MTAD
   IRES
   PhyB(1-621)-SV40NLS-Gal4DBD
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   HA tag and SV40NLS

   Expression

   Mammalian

   Mutation

   pif3 1-523

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NSP2-Flag

   Plasmid

   #210344

   Purpose

   Expresses SARS-CoV-2 NSP2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BNLS

   Plasmid

   #50836

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP B
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-mito-LAR-GECO1.2

   Plasmid

   #61245

   Purpose

   Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1.2
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1.2: N45I/A47R/E1…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-hIes2

    Plasmid

    #15355

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    PAP-1 binding protein (INO80B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NSP14-Flag

    Plasmid

    #210346

    Purpose

    Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FN-Ets2

    Plasmid

    #44603

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 (Ets2 Mouse)
    

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-hCGN

    Plasmid

    #236507

    Purpose

    For expression of Cingulin (human) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGN (CGN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hCGN-HA

    Plasmid

    #236508

    Purpose

    For expression of Cingulin (human) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGN (CGN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT-C34-L

    Plasmid

    #223967

    Purpose

    Anti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NSP4-FR-2Strep

    Plasmid

    #210316

    Purpose

    Expresses SARS-CoV-2 NSP4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. S113

    Plasmid

    #174551

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    mdm2 and designed protein LD6 (MDM2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge