We narrowed to 171,128 results for: addgene

Showing: 5281 - 5320 of 171128 results

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5.1kh

Plasmid

#160297

Purpose

Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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WT-Cas9

Plasmid

#239382

Purpose

For circular RNA-mediated inverse prime editor using WTCas9 in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

Cas9

Use

CRISPR

Tags

BPNLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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iPE-WT-Cas9

Plasmid

#239379

Purpose

For inverse prime editor using WTCas9 in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

Cas9, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_BFP-P2A-NTRv2-pA

Plasmid

#200538

Purpose

mini circle middle entry plasmid for BFP-P2A-NTR version 2-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-3 without ATG

Plasmid

#200519

Purpose

mini circle middle entry plasmid for 2a-eGFP-pA ORF3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-1 with ATG

Plasmid

#200522

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-2 with ATG

Plasmid

#200523

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-3 with ATG

Plasmid

#200524

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-1 with ATG

Plasmid

#200514

Purpose

mini circle middle entry plasmid for 2a-eGFP-pA ORF1 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-2 with ATG

Plasmid

#200515

Purpose

mini circle middle entry plasmid for 2a-GFP-pA ORF 2 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-3 with ATG

Plasmid

#200516

Purpose

mini circle middle entry plasmid for 2a-GFP-pA-ORF 3 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-1 without ATG

Plasmid

#200517

Purpose

mini circle middle entry plasmid for 2a-eGFP-pA ORF1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-2 without ATG

Plasmid

#200518

Purpose

mini circle middle entry plasmid for 2a-eGFP-pA ORF2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM26

Plasmid

#233498

Purpose

Level 0 plasmid for MoClo, encodes the TDH3 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promTDH3

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM27

Plasmid

#233499

Purpose

Level 0 plasmid for MoClo, encodes the HFF1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promHFF1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM28

Plasmid

#233500

Purpose

Level 0 plasmid for MoClo, encodes the NC1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promNC1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM29

Plasmid

#233501

Purpose

Level 0 plasmid for MoClo, encodes the COX20 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promCOX20

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM22

Plasmid

#233494

Purpose

Level 0 plasmid for MoClo, encodes the INU1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promINU1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM23

Plasmid

#233495

Purpose

Level 0 plasmid for MoClo, encodes the ADH1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promADH1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM24

Plasmid

#233496

Purpose

Level 0 plasmid for MoClo, encodes the PGK promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promPGK1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM25

Plasmid

#233497

Purpose

Level 0 plasmid for MoClo, encodes the TEF3 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promTEF3

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM09

Plasmid

#233481

Purpose

Level 0 plasmid for MoClo, encodes the PST1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promPST1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM10

Plasmid

#233482

Purpose

Level 0 plasmid for MoClo, encodes the GLK1A promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promGLK1A

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM11

Plasmid

#233483

Purpose

Level 0 plasmid for MoClo, encodes the GLK1B promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promGLK1B

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM12

Plasmid

#233484

Purpose

Level 0 plasmid for MoClo, encodes the SCL1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promSCL1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM13

Plasmid

#233485

Purpose

Level 0 plasmid for MoClo, encodes the ZWF promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PromZWF

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM14

Plasmid

#233486

Purpose

Level 0 plasmid for MoClo, encodes the RPE1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promRPE1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM15

Plasmid

#233487

Purpose

Level 0 plasmid for MoClo, encodes the GPD1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promGPD1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM16

Plasmid

#233488

Purpose

Level 0 plasmid for MoClo, encodes the ALD2 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promALD1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM17

Plasmid

#233489

Purpose

Level 0 plasmid for MoClo, encodes the HSP26 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promHSP26

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM18

Plasmid

#233490

Purpose

Level 0 plasmid for MoClo, encodes the HSP60 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promHSP60

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM19

Plasmid

#233491

Purpose

Level 0 plasmid for MoClo, encodes the PIR1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promPIR1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM20

Plasmid

#233492

Purpose

Level 0 plasmid for MoClo, encodes the SSA3 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promSSA3

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM21

Plasmid

#233493

Purpose

Level 0 plasmid for MoClo, encodes the SOD1 promoter for K.marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

promSOD1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM01

Plasmid

#233473

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTKM03

Plasmid

#233475

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235325

Purpose

PiggyBac integration vector for ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20-Src(E378G)

Plasmid

#215466

Purpose

Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Lentiviral

Mutation

E378G (constitutively-active)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHR-Flag-IF1-Fibcon-icam

Plasmid

#233076

Purpose

expresses synCAM -IF1-fibcon - iCAM1

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

If1-Icam

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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