3,636 results

1. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

   Plasmid

   #146013

   Purpose

   Insect Expression of DmSmaug_763-999

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_763-999 (smg Fly)
   

   Expression

   Insect

   Mutation

   2 silent mutations and a deletion of the AA 961-9…

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmTral-R42E_B

   Plasmid

   #145988

   Purpose

   Insect Expression of DmTral-R42E

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42E (tral Fly)
   

   Expression

   Insect

   Mutation

   two silent mutations A198C, A387G and a mutation …

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

   Plasmid

   #146001

   Purpose

   Insect Expression of DmSmaug_1-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_1-762 (smg Fly)
   

   Tags

   V5-6xHis

   Expression

   Insect

   Mutation

   NP_523987.1, 7 silent mutations compared to the s…

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-lambdaN-HA-DmZuc_B

   Plasmid

   #145949

   Purpose

   Insect Expression of DmZuc

   Depositor

   Elisa Izaurralde

   Insert

   DmZuc (zuc Fly)
   

   Expression

   Insect

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmExu_B

   Plasmid

   #145939

   Purpose

   Insect Expression of DmExu

   Depositor

   Elisa Izaurralde

   Insert

   DmExu (exu Fly)
   

   Expression

   Insect

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

   Plasmid

   #145898

   Purpose

   Insect Expression of DmAGO1-F629V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   DmAGO1-F629V (AGO1 Fly)
   

   Expression

   Insect

   Mutation

   V315A, F629V, H913R - variation with 2 non silent…

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmLSm3_A

   Plasmid

   #145888

   Purpose

   Insect Expression of DmLSm3

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   DmLSm3 (LSm3 Fly)
   

   Expression

   Insect

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-EGFP-DmDCP2_209-791_A

   Plasmid

   #145848

   Purpose

   Insect Expression of DmDCP2_209-791

   Depositor

   Elisa Izaurralde

   Insert

   DmDCP2_209-791 (DCP2 Fly)
   

   Expression

   Insect

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3. 3xFlag:PP4-19c[D85NH115N]

   Plasmid

   #112247

   Purpose

   expression of fly mutant PP4

   Depositor

   Simone Di Giovanni

   Insert

   PP4 (Pp4-19C Fly)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D85NH115N

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AByH

    Plasmid

    #164152

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AByD

    Plasmid

    #164150

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AByC

    Plasmid

    #164149

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AByB

    Plasmid

    #164148

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AByA

    Plasmid

    #163749

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

    Plasmid

    #163927

    Purpose

    Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

    Plasmid

    #163925

    Purpose

    Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
    

    Use

    Cre/Lox

    Tags

    tagBFP

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 908I

    Plasmid

    #160241

    Purpose

    VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Van Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 907F1

    Plasmid

    #160233

    Purpose

    FlightN-VanR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    VanR
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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