3,636 results

1. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

   Plasmid

   #146001

   Purpose

   Insect Expression of DmSmaug_1-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_1-762 (smg Fly)
   

   Use

   Tags

   V5-6xHis

   Expression

   Insect

   Mutation

   NP_523987.1, 7 silent mutations compared to the s…

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-DmZuc_B

   Plasmid

   #145949

   Purpose

   Insect Expression of DmZuc

   Depositor

   Elisa Izaurralde

   Insert

   DmZuc (zuc Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmExu_B

   Plasmid

   #145939

   Purpose

   Insect Expression of DmExu

   Depositor

   Elisa Izaurralde

   Insert

   DmExu (exu Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

   Plasmid

   #145898

   Purpose

   Insect Expression of DmAGO1-F629V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   DmAGO1-F629V (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   V315A, F629V, H913R - variation with 2 non silent…

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmLSm3_A

   Plasmid

   #145888

   Purpose

   Insect Expression of DmLSm3

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   DmLSm3 (LSm3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-EGFP-DmDCP2_209-791_A

   Plasmid

   #145848

   Purpose

   Insect Expression of DmDCP2_209-791

   Depositor

   Elisa Izaurralde

   Insert

   DmDCP2_209-791 (DCP2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3. 3xFlag:PP4-19c[D85NH115N]

   Plasmid

   #112247

   Purpose

   expression of fly mutant PP4

   Depositor

   Simone Di Giovanni

   Insert

   PP4 (Pp4-19C Fly)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D85NH115N

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AByH

   Plasmid

   #164152

   Purpose

   Integrates marker gene onto D. melanogaster Y chromosome

   Depositor

   Omar Akbari

   Article

   Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

   Insert

   Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AByD

   Plasmid

   #164150

   Purpose

   Integrates marker gene onto D. melanogaster Y chromosome

   Depositor

   Omar Akbari

   Article

   Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

   Insert

   Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AByC

    Plasmid

    #164149

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AByB

    Plasmid

    #164148

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AByA

    Plasmid

    #163749

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Insert

    Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

    Plasmid

    #163927

    Purpose

    Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

    Plasmid

    #163925

    Purpose

    Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
    

    Use

    Cre/Lox

    Tags

    tagBFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 908I

    Plasmid

    #160241

    Purpose

    VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Van Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 907F1

    Plasmid

    #160233

    Purpose

    FlightN-VanR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    VanR
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

    Plasmid

    #159631

    Purpose

    expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

    Depositor

    Andrew Allen, Ross Bathgate

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    Ast (AstA Fly)
    

    Use

    Lentiviral

    Tags

    HA, IRES, mCherry, and neurophysin-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OA-1045B

    Plasmid

    #125004

    Purpose

    Expresses gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge