We narrowed to 7,122 results for: ef1a

1. pSLCAR-CD19-CD3z

   Plasmid

   #135993

   Purpose

   Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-3z-P2A-EGFP (CD247, CD28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-mTubb3

   Plasmid

   #87116

   Purpose

   AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ai14-HITI

   Plasmid

   #87117

   Purpose

   AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-hCCND1-T286A-HA

   Plasmid

   #174155

   Purpose

   Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   A>G at base 856 (Thr>Ala at amino acid 286)

   Promoter

   EF1A

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB-AMBRA1-501*-3xFLAG

   Plasmid

   #174160

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 501-1208

   Promoter

   EF1A

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPB-AMBRA1-217*-3xFLAG

   Plasmid

   #174161

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 217-1208

   Promoter

   EF1A

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

   Plasmid

   #115891

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α promoter (1.1kb short version)

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMaster1

   Plasmid

   #58525

   Purpose

   Reprogram somatic cells to iPS cells

   Depositor

   Mario Capecchi

   Article

   Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

   Inserts

   neo
   tk
   oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 )
   myc (MYC Human)
   EBNA-1
   Amp
   Lin28 (LIN28A Human)
   Nanog (NANOG Human)
   IRES
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAG and EF1a

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BII-ChPtW-tdT

    Plasmid

    #133401

    Purpose

    Piggybac vector for constitutive expression of tdTomato

    Depositor

    Blair Madison

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BII-ChPtW-iRFP702

    Plasmid

    #133398

    Purpose

    Piggybac vector for constitutive expression of iRFP702

    Depositor

    Blair Madison

    Insert

    IRFP-702
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BII-ChPtW-iRFP670

    Plasmid

    #133397

    Purpose

    Piggybac vector for constitutive expression of iRFP670-NLS

    Depositor

    Blair Madison

    Insert

    iRFP-670-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BII-ChPtW-smURFP

    Plasmid

    #133399

    Purpose

    Piggybac vector for constitutive expression of smURFP-NLS

    Depositor

    Blair Madison

    Insert

    smURFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BII-Sh-BnTW

    Plasmid

    #133365

    Purpose

    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    Flag-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBbi-pur H-2Kb

    Plasmid

    #111623

    Purpose

    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

    Depositor

    Jon Yewdell

    Insert

    H2Kb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid

    #20949

    Purpose

    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    halorhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1-MH.Bl-mDcrSOM

    Plasmid

    #120540

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    Dicer (Dicer1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF1-MH.Bl-mDcrOO

    Plasmid

    #120541

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    Dicer (Dicer1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p16L1-GFP

    Plasmid

    #89910

    Purpose

    Self-packages in TT-16L2 stable line. L1-only capsid propagation.

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2005 Mar;79(5):2839-46.

    Insert

    HPV16L1+ GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    SV40 and hEF1alpha

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCS4077

    Plasmid

    #131738

    Purpose

    pLenti_CMV-mCherry-BghA_EF1alpha-BFP

    Depositor

    Christina Smolke

    Article

    Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid

    #188722

    Purpose

    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein

    Depositor

    Darrell Kotton

    Insert

    TFEB (TFEB Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    hSP-C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Aiolos ZF2 (AA146-168) in Cilantro 2

    Plasmid

    #74456

    Purpose

    Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SOX17-tdTomato-EhB

    Plasmid

    #210467

    Purpose

    Donor plasmid for knock-in tdTomato into the human SOX17 locus

    Depositor

    Kai Wang

    Insert

    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJA317

    Plasmid

    #74488

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d1ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJA318

    Plasmid

    #74489

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d4ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMpGWB103-Citrine

    Plasmid

    #79137

    Purpose

    Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

    Depositor

    Yutaka Kodama

    Article

    Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

    Insert

    Citrine
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    MpEF1a promoter

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-EGFP-hChibby1

    Plasmid

    #89770

    Purpose

    Labels mother centrioles/basal bodies

    Depositor

    Ken-Ichi Takemaru

    Insert

    Chibby1 (CBY1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. aCE120

    Plasmid

    #204491

    Purpose

    Figure 1. reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    mKate2
    EYFP
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. aCE121

    Plasmid

    #204492

    Purpose

    Cadherin circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    Cdh6-mKate2
    Cdh1-EYFP
    

    Use

    Synthetic Biology

    Tags

    2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEBB-BT

    Plasmid

    #36295

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Type

    Empty backbone

    Use

    Tags

    BT-tag (peptide sequence from P.freudenreichii tr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSL1045

    Plasmid

    #129525

    Purpose

    pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    dSpCas9 (Other)
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJTT002

    Plasmid

    #204493

    Purpose

    Reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    TagBFP
    EYFP
    mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LacZ_pHIVEGFP

    Plasmid

    #191251

    Purpose

    Expresses LacZ in mammalian cells (used as a control)

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

    Insert

    LacZ
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEF HaloTag-SYT1

    Plasmid

    #197280

    Purpose

    Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEF1α-Cre-2A-mScarlet

    Plasmid

    #207745

    Purpose

    Expression of the Cre recombinase linked to a mScarlet fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mScarlet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pHEFI-OptoCARCD19-mScarlet

    Plasmid

    #170465

    Purpose

    Expresses an optically-controllable Chimeric antigen receptor that binds CD19 antigen, fused to mScarlet fluorophore

    Depositor

    John James

    Article

    Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

    Insert

    OptoCAR-CD19
    

    Use

    Lentiviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEF1α-Cre-2A-moxGFP

    Plasmid

    #207744

    Purpose

    Expression of the Cre recombinase linked to a moxGFP fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-moxGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pEF1α-Cre-2A-mTagBFP2

    Plasmid

    #207746

    Purpose

    Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mTagBFP2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. vYM140_PB-pEFa1-iaaM(P.s)-Hygro

    Plasmid

    #160043

    Purpose

    Expressing iaaM for converting L-Trp to IAM

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    iaaM (iaaM )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PB-T3G-CERP2

    Plasmid

    #175498

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHS-435

    Plasmid

    #195470

    Purpose

    Lentiviral Integratable Constitutive super IL-2 cytokine

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pEF1a-Super IL-2-2A-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEF1α-Cre-2A-miRFP670nano3

    Plasmid

    #207747

    Purpose

    Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-miRFP670nano3
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pEF SYT1-HaloTag

    Plasmid

    #197281

    Purpose

    Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EF1alpha

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. PB-T3G-ERP2

    Plasmid

    #175188

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge