We narrowed to 15,903 results for: grna

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2

Plasmid

#80275

Purpose

GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Publication

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Publication

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Publication

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Expression

Mammalian

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pK-Cas9-tracr-L

Plasmid

#230860

Purpose

Long tracrRNA (tracr-L) from Streptococcus pyogenes which acts as a natural single guide RNA

Depositor

Kutubuddin Molla

Publication

Karmakar et al Plant Cell Rep. 2024 Nov 23;43(12):291.

Insert

Long tracrRNA (tracr-L) from S. pyogenes replaces gRNA scaffold

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available since

July 15, 2026

Availability

Academic Institutions and Nonprofits only

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pDIV1064

Plasmid

#247972

Purpose

CRISPR-Cas9 vector containing two 20 nt protospacer sequences to target the gene wA in Aspergillus nidulans. pyrG marker

Depositor

Uffe Mortensen

Insert

Cas9-AMA1-wA-gRNA 1 & 2-pyrG-Ori-AmpR

Use

CRISPR

Mutation

none

Available since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-1-EF1a-LibVec

Plasmid

#239592

Purpose

expresses non-targeting (NT) guide#1

Depositor

Andreas Moor

Publication

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

Plasmid

#210734

Purpose

Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp D10A Cas9
Frodo sgCAG

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13220

Plasmid

#219895

Purpose

The base plasmid of TUNEYALI for TF30

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30

Expression

Yeast

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13237

Plasmid

#219910

Purpose

The base plasmid of TUNEYALI for TF47

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47

Expression

Yeast

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13204

Plasmid

#219879

Purpose

The base plasmid of TUNEYALI for TF14

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14

Expression

Yeast

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13242

Plasmid

#219914

Purpose

The base plasmid of TUNEYALI for TF52

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52

Expression

Yeast

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13245

Plasmid

#219916

Purpose

The base plasmid of TUNEYALI for TF55

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55

Expression

Yeast

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13193

Plasmid

#219868

Purpose

The base plasmid of TUNEYALI for TF03

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03

Expression

Yeast

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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