We narrowed to 15,088 results for: pET

1. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

   Plasmid

   #169659

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-SU(Z)12(624-900)-H6

   Plasmid

   #183772

   Purpose

   Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

   Insert

   SU(Z)12 residues 624-900 (Su(z)12 Fly)
   

   Tags

   His tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM-60-NusA-Dmp68_B

   Plasmid

   #145966

   Purpose

   Bacterial Expression of Dmp68

   Depositor

   Elisa Izaurralde

   Insert

   Dmp68 (Rm62 Fly)
   

   Expression

   Bacterial

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a Cdk2ap1ΔN (MT2B2)

   Plasmid

   #178034

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   T7LacI

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-CPL2/pET28a

   Plasmid

   #172095

   Purpose

   cDNA of CER11/CPL2 cloned into pET28a for protein expression

   Depositor

   George Haughn

   Article

   Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

   Insert

   CPL2 (CPL2 Mustard Weed)
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1delZn3-L713F

   Plasmid

   #173934

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-G871L

   Plasmid

   #174798

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G871L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G871L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-A870L

   Plasmid

   #174797

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A870L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A870L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-K893I

    Plasmid

    #174799

    Purpose

    Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-K893I (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K893I, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET41sIII_GST-ALC1-K77R

    Plasmid

    #174813

    Purpose

    Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1-K77R (CHD1L Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K77R

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET41sIII_GST-ALC1-E175Q

    Plasmid

    #174814

    Purpose

    Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1-E175Q (CHD1L Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E175Q

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-43

    Plasmid

    #172759

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-43
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-35

    Plasmid

    #172755

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-35
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-19

    Plasmid

    #172748

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-19
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-3

    Plasmid

    #172740

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-12

    Plasmid

    #172744

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-12
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-14

    Plasmid

    #172745

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-14
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-11a HIV-1 P6

    Plasmid

    #154201

    Purpose

    Bacterial expression of Gag P6 from HIV-1 HXB2 strain

    Depositor

    Lalit Deshmukh

    Article

    Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

    Insert

    Gag P6 protein from HIV-1 clone HXB2
    

    Tags

    GB1-6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-DEST42-dre4

    Plasmid

    #170440

    Purpose

    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    dre4 (dre4 Fly)
    

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-20

    Promoter

    T7

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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