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Plasmid#225613PurposeReplicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrineDepositorInsertmCitrine
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-SpRYn-Act3.0
Plasmid#178958PurposeIt consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-SpRYn-Act3.0
Plasmid#178959PurposeIt consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBP_2xNbLYS1::LUZ
Plasmid#189918PurposeBinary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.DepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterVariousAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKY925F
Plasmid#216547PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: Y925F ) of human PTK2DepositorInsertPTK2 (Y925F) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation Y925FPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC4
Plasmid#91182PurposeT-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting 58kb region in medicago truncatula
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC2
Plasmid#91180PurposeT-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting NCR genes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC362
Plasmid#91216Purposeprotoplast vector for targeted deletion of 6 genes in tomatoDepositorInsertgRNAs targeting 6 tomato genes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC3
Plasmid#91181PurposeT-DNA vector for targeted deletion of 58kb region in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting 58kb region in medicago truncatula
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-224_Cacnes_sfGFP
Plasmid#225611PurposeReplicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.DepositorInsertsfGFP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-225_Cacnes_mKO2
Plasmid#225612PurposeReplicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2DepositorInsertmKO2
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS722A
Plasmid#216545PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S722A) of human PTK2DepositorInsertPTK2 (S722A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S722APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKY861F
Plasmid#216546PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2DepositorInsertPTK2 (Y861F) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation Y861FPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKY397F
Plasmid#216677PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: Y397F) of human PTK2DepositorInsertPTK2 (Y397F) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation Y397FPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS732A
Plasmid#216541PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2DepositorInsertPTK2 (S732A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S732APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS910A
Plasmid#216542PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2DepositorInsertPTK2 (S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S910APromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN381 - pTRV2-CHLH
Plasmid#203886PurposeViral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.DepositorInsertCHLH fragment
UseRNAiTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only