We narrowed to 11,272 results for: VARS

1. pDest17_A1-LCD swap X9

   Plasmid

   #226551

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X9

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X9
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDest17_A1-LCD swap X8

   Plasmid

   #226550

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X8

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X8
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDest17_A1-LCD swap X6

   Plasmid

   #226548

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X6

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X6
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDest17_A1-LCD swap X2

   Plasmid

   #226544

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X2

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X2
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBJT031

   Plasmid

   #225177

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBJT034

   Plasmid

   #225180

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBJT032

   Plasmid

   #225178

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBJT033

   Plasmid

   #225179

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHBJT030

   Plasmid

   #225176

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBJT004

    Plasmid

    #225155

    Purpose

    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHBJT003

    Plasmid

    #225154

    Purpose

    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LAMTOR1-APEX2

    Plasmid

    #215556

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMTOR1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCL194

    Plasmid

    #222225

    Purpose

    Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    site 1/Cas9/Eco1 RT
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCL195

    Plasmid

    #222226

    Purpose

    Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    site 2/Cas9/Eco1 RT
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCL368

    Plasmid

    #222227

    Purpose

    Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    site 3/Cas9/Eco1 RT
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_PA1472_Cterm_His

    Plasmid

    #220485

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_speG_Nterm_His

    Plasmid

    #220478

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_PA4114_Nterm_His

    Plasmid

    #220479

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_PA1472_Nterm_His

    Plasmid

    #220480

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_PA1377_Nterm_His

    Plasmid

    #220481

    Purpose

    heterologous expression of PA1377 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1377
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge