We narrowed to 5,822 results for: pCas

1. pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108806

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

   Plasmid

   #108795

   Purpose

   Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa571
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

   Plasmid

   #108796

   Purpose

   Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa572 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FKBP and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

   Plasmid

   #101177

   Purpose

   Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

   Plasmid

   #108769

   Purpose

   Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment N-terminus to aa396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

   Plasmid

   #36881

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   tdT-3Myc
   insulator
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

   Plasmid

   #101205

   Purpose

   Bacterial expression plasmid for SpCas9 REC3 domain

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K506–Q712
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K506–Q712

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

   Plasmid

   #108848

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

    Plasmid

    #108846

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

    Plasmid

    #100979

    Purpose

    Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    c-Myc NLS-FKBP-Venus N-T2A-mRFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

    Plasmid

    #108737

    Purpose

    Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa270
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

    Plasmid

    #87560

    Purpose

    Expresses aa397-423 of Flp recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO aa397-423
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    PYL

    Expression

    Mammalian

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #87559

    Purpose

    Expresses aa1-396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO aa1-396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

    Plasmid

    #108773

    Purpose

    Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa269
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

    Plasmid

    #108774

    Purpose

    Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa270 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

    Plasmid

    #108840

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

    Plasmid

    #108836

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA

    Plasmid

    #108842

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GAI,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge