We narrowed to 2,365 results for: DAP

1. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-FLAG-p14

   Plasmid

   #42330

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p14 (LAMTOR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IBU-pSu9-HaloTag7-SNAP

   Plasmid

   #217319

   Purpose

   Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

   Depositor

   Noboru Mizushima

   Article

   Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

   Insert

   Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
   

   Use

   Retroviral

   Tags

   HaloTag and SNAP

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmiRGLO Dual-Luciferase-MYB-3'UTR

   Plasmid

   #182250

   Purpose

   Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of MYB gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-AAVS1-Ef1a-Puro-moxGFP

   Plasmid

   #227273

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-FADD

   Plasmid

   #78802

   Purpose

   To overexpress FADD in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

   Insert

   FADD (FADD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AP-1 mu1-GFP

    Plasmid

    #173432

    Purpose

    Expression of AP-1 Mu1 subunit with a terminal GFP

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP1M1 (Ap1m1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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