We narrowed to 3,800 results for: golden gate

1. RCIscript-GoldyTALEN

   Plasmid

   #38142

   Purpose

   destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

   Depositor

   Stephen Ekker, Daniel Carlson

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Mrna transcription vector

   Tags

   AcV5 and FokI homodimer

   Mutation

   Truncate at N and C terminous

   Promoter

   T3

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGY037 BSMV-gamma-AmCyan

   Plasmid

   #234818

   Purpose

   To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   AmCyan
   

   Expression

   Plant

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL3478 PB-PEmax-mTagBFP2

   Plasmid

   #226664

   Purpose

   PiggyBac cargo vector encoding PEmax and mTagBFP2

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Inserts

   PEmax
   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG and UBC

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL90002

   Plasmid

   #68261

   Purpose

   Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter: U6-26 (Arabidopsis thaliana)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB036 FoMV-sgGFP

   Plasmid

   #234813

   Purpose

   To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting GFP
   

   Expression

   Plant

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-BLU-VIPR

   Plasmid

   #220497

   Purpose

   Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

   Depositor

   Alexander Espinosa

   Article

   Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLU-VIPR

   Plasmid

   #220498

   Purpose

   Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

   Depositor

   Alexander Espinosa

   Article

   Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Expression

   Mammalian

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PDCD1-DEM (P1#5)

   Plasmid

   #227738

   Purpose

   Designer Epigenome Modifier (DEM) designed to target the PDCD1-promoter

   Depositor

   Claudio Mussolino

   Article

   Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

   Insert

   Designer epigenome modifier targeting PDCD1-promoter
   

   Use

   TALEN

   Tags

   DNMT3A, DNMT3L, KRAB

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT137-splitC

    Plasmid

    #122605

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-Puro-24xMS2

    Plasmid

    #212629

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT137-splitD

    Plasmid

    #122606

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTU2S-a (p15A origin)

    Plasmid

    #74088

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT100.106b

    Plasmid

    #122601

    Purpose

    clone APOBEC1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    APOBEC1
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL90003

    Plasmid

    #68262

    Purpose

    Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6 (Triticum aestivum)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT13-splitC

    Plasmid

    #122596

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZA26

    Plasmid

    #158504

    Purpose

    In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc (ZAR1 Mustard Weed)
    

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    D489V

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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