We narrowed to 6,089 results for: nls

1. pCR1063

   Plasmid

   #111091

   Purpose

   Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   dCas9-KRAB
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. A3A-PBE-ΔUGI

   Plasmid

   #119770

   Purpose

   Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-ΔUGI-NLS
   

   Use

   Tags

   NLS

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM2-rPMCA2a

   Plasmid

   #47762

   Purpose

   mammalian expression of rat PMCA2a

   Depositor

   Emanuel Strehler, John Penniston

   Article

   Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6

   Insert

   PMCA2a (Atp2b2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET42b-A3A-PBE-ΔUGI

   Plasmid

   #119771

   Purpose

   To express the recombinant APOBEC3A in vitro

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS
   

   Use

   Tags

   6*HIS and NLS

   Expression

   Bacterial

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   T7 promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PlmdCas12e KRAB

   Plasmid

   #188506

   Purpose

   Expresses FLAG tagged PlmdCas12e KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12e
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-FLAG-KRAB-NLS

   Expression

   Mammalian

   Mutation

   D659A/E756A/D922A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPyCAG-NE-EGFP-IRES-Pac

   Plasmid

   #183617

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

   Insert

   EGFP
   

   Use

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RNAPII_CTD27-52_K5S

   Plasmid

   #98679

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K1838S, K1859S, K1866S, K1873S, K1887S

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RNAPII_CTD38-52

    Plasmid

    #98685

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    heptads 38-52, residues 1853-1970

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMGF173

    Plasmid

    #96999

    Purpose

    NLS-GFP-lacZ leu1 insertion vector

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    lacZ
    

    Use

    Tags

    NLS-GFP

    Expression

    Yeast

    Mutation

    Promoter

    Pnmt1

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. kank2_pJet1.2

    Plasmid

    #121980

    Purpose

    for generating kank2 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank2 (LOC571549 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-myc-mCE(K294A)

    Plasmid

    #82461

    Purpose

    Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mCE (K294A) (Rngtt Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Lysine 294 changed to Alanine

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCR1277

    Plasmid

    #111101

    Purpose

    Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51D gRNA (RAD51D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RNAPII_CTD27-37

    Plasmid

    #98686

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    heptads 27-37, residues 1773-1852

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-myc NES- mCE(K294A)

    Plasmid

    #82464

    Purpose

    Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt Mouse, HIV)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K294A

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.0_nucLAMA-G97 IRES mYFP

    Plasmid

    #130713

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.0_nucLAMA-F98 IRES mYFP

    Plasmid

    #130712

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. kank4_pJet1.2

    Plasmid

    #121982

    Purpose

    for generating kank4 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank4 (kank4 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RNAPII_CTD43-52

    Plasmid

    #98684

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hepatds 43-52, residues 1888-1970

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge