We narrowed to 18,759 results for: nls
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Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT23_GalL_BE-PAPAP
Plasmid#143486PurposeExpresses BE-PAPAP in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ARB342
Plasmid#124025PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreenDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB343
Plasmid#124026PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLS-A4_EF1a-NLS::mRuby2-bgPA_R1
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNXRVd-HACre
Plasmid#78404Purposeconstitutive Cre expression vectorDepositorInsertsExpressionYeastAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-EGFR NLS zeo
Plasmid#192337PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-EGFR NLSDepositorAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-EGFR NLS
Plasmid#192315PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLSDepositorAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-NLS-BirA-2A-mCherry_Ras
Plasmid#80065PurposeZic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X2-RalBP1
Plasmid#37245DepositorAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega1_[10x 2EBS-S10:35Smp:NLS:YPet:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254064PurposeYPet expression driven by 10x 2EBS-S10DepositorInsert10x 2EBS-S10:35Smp:NLS:YPet:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceAug. 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega1_[mEBSn:mini35S:3x SV40-NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254070PurposeGUS expression driven by mEBSnDepositorInsertmEBSn:mini35S:3x SV40-NLS:GUS:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega2_[10x 2EBS-S10:35Smp:NLS:GUS:35Sterm]-[tNOS:BASTA:pNOS]
Plasmid#254066PurposeGUS expression driven by 10x 2EBS-S10DepositorInsert10x 2EBS-S10:35Smp:NLS:GUS:35Sterm
UseSynthetic BiologyExpressionPlantAvailable sinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194890PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1
UseLentiviralTagsHAMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194894PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194886PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
Tags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cLYN268
Plasmid#188259PurposePlasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcLYN268 (LYN Human)
UseCRISPRExpressionMammalianAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:mScarletNLS-SV40pA
Plasmid#155124PurposeTol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:mScarletNLS
UseZebrafish expressionPromoterQUASM1-E1bAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
Plasmid#219537Purposea. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotorDepositorAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG
Plasmid#86871PurposeTo express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSF3B6 (SF3B6 Human)
TagsEGFP and FLAGExpressionMammalianMutationamino acids 7-29 deletionPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only