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pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

Plasmid

#179902

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

antiGFPnanobody-mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.3 K+ channel [N375/67R]

Plasmid

#177537

Purpose

Mammalian Expression Plasmid of anti-Kv3.3 K+ channel (Mouse). Derived from hybridoma N375/67.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.3 K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

Plasmid

#105194

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-1Pe

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

Plasmid

#105195

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(LC)-1Pe

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (P.1 lineage / Brazilian variant)

Plasmid

#169847

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Use

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (B.1.1.7 lineage / UK variant)

Plasmid

#169848

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Use

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kir2.1 K+ channel [N112B/14R]

Plasmid

#177496

Purpose

Mammalian Expression Plasmid of anti-Kir2.1 K+ channel (Mouse). Derived from hybridoma N112B/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir2.1 K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnj2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.4 K+ channel (extracellular) [L71/5R]

Plasmid

#177488

Purpose

Mammalian Expression Plasmid of anti-Kv1.4 K+ channel (extracellular) (Rat). Derived from hybridoma L71/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.4 K+ channel [N72/16R]

Plasmid

#177568

Purpose

Mammalian Expression Plasmid of anti-Kv3.4 K+ channel (Rat). Derived from hybridoma N72/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv3.4 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.4/KCNQ4 K+ channel [N43/6R]

Plasmid

#177552

Purpose

Mammalian Expression Plasmid of anti-Kv7.4/KCNQ4 K+ channel (Human). Derived from hybridoma N43/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.4/KCNQ4 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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