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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,180 results for: pcDNA3.1+

Showing: 4141 - 4170 of 5180 results

  1. LUC05 (L/Cys/P)

    Plasmid
    #112146
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112146-plasmid-map-sequence-id-214888

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  16. Rlucrarr3-Flash6

    Plasmid
    #74134
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  19. CoV2-N-R203K

    Plasmid
    #177951
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (R203K) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R203K
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177951-plasmid-map-sequence-id-348191

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  21. Rlucrarr3-Flash1

    Plasmid
    #74129
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. Rlucrarr3-Flash4

    Plasmid
    #74132
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. Rlucrarr3-Flash3

    Plasmid
    #74131
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. Rlucrarr3-Flash2

    Plasmid
    #74130
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 4141 - 4170 of 5180 results