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FH95pUP95W (E6)

Plasmid

#74036

Purpose

lentiviral expression of Psd95 shRNA and Psd95 (no GFP)

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
His-myc-Med20 pBacPAK8

Plasmid

#15370

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

Med20

Use

Baculovirus construction

Tags

His and myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pSicoR-UBE2D3i1

Plasmid

#59586

Purpose

Expression of shRNA against human UBE2D3

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

UBE2D3 (UBE2D3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PLCE1i2

Plasmid

#59629

Purpose

Expression of shRNA against human PLCE1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PLCE1 (PLCE1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5_TO_FRT_myc_GLUT4_2Ala_APEX2

Plasmid

#250767

Purpose

Mammalian cell expression of GLUT4 in which L500 and L503 are mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(2Ala)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Mutation

L500 and L503 mutated to alanines

Promoter

CMV

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250765

Purpose

Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

VPS35 shRNA

Tags

SSF-SNAP(for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_2Ala_APEX2

Plasmid

#250774

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pFY20

Plasmid

#253057

Purpose

low copy episomal cloning vector pFY20 for use in fission yeast

Depositor

Mari Davidson, Wayne Wahls

Article

Wahls et al Yeast. 2008 Sep;25(9):643-50. doi: 10.10

Type

Empty backbone

Use

Low copy number, episomal cloning vector

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BubR1-D911A

Plasmid

#200815

Purpose

expresses BubR1-D911A in mammalian cells

Depositor

Hung-Chun Chang

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Aspartic acid 911 to Alanine

Promoter

CAG

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGH1110

Plasmid

#223682

Purpose

U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm8.1

Plasmid

#210288

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.0

Plasmid

#210300

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Vector 1174K

Plasmid

#221017

Purpose

Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv [Preprint]. 2024 Jul 18:2024.07.

Insert

Engineered dobulesex splicing module (Anopheles gambiae)

Use

Synthetic Biology

Expression

Insect

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-PlmCasX-T2A-EGFP

Plasmid

#188273

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-6xbsMS2-8xlet7_AC

Plasmid

#148433

Purpose

Mammalian Expression of 6xbsMS2-8xlet7

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-8xlet7

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-TISU-RLuc-6xbsMS2_AC

Plasmid

#148434

Purpose

Mammalian Expression of 6xbsMS2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-TISU-RLuc-6xbsMS2-8xlet7_AC

Plasmid

#148435

Purpose

Mammalian Expression of 6xbsMS2-8xlet7

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-8xlet7

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-TISU-RLuc-6xbsMS2-random_AC

Plasmid

#148437

Purpose

Mammalian Expression of 6xbsMS2-random

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-random

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TISU-HA-hRLuc-V5-6xbsMS2_AC

Plasmid

#148424

Purpose

Mammalian Expression of TISU-HA-hRluc-V5-6xbsMS2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

TISU-HA-hRluc-V5-6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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