We narrowed to 2,785 results for: cdna expression plasmid

1. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

   Plasmid

   #185702

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(BCA)
   

   Use

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Kv1.2 K+ channel scFv [K14/16]

   Plasmid

   #182064

   Purpose

   Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Kv2.1 K+ channel scFv [K89/34]

   Plasmid

   #182069

   Purpose

   Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K89/34.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Mortalin/GRP75 scFv [N52A/42]

   Plasmid

   #182091

   Purpose

   Mammalian Expression of Mortalin/GRP75 scFv. Derived from hybridoma N52A/42.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Mortalin/GRP75 (Mus musculus) (Hspa9 Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

   Plasmid

   #159630

   Purpose

   AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

   Depositor

   Andrew Allen, Ross Bathgate

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   AAV

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Mutation

   Promoter

   DCA (same as CAG promoter)

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

   Plasmid

   #185488

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

   Plasmid

   #185485

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

   Plasmid

   #185484

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

   Plasmid

   #185486

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

    Plasmid

    #185487

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lucMAPT-30D

    Plasmid

    #214672

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lucMAPT-30U

    Plasmid

    #214673

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lucMAPT-GenRep

    Plasmid

    #214674

    Purpose

    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRUPATH Triple Gaq

    Plasmid

    #196058

    Purpose

    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRUPATH Triple GaoA

    Plasmid

    #196051

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRUPATH Triple Ga13

    Plasmid

    #196062

    Purpose

    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRUPATH Triple Gai3

    Plasmid

    #196050

    Purpose

    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge