Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 62,913 results for: cloning

Clear keywords
Showing: 2761 - 2780 of 62913 results

  1. p5'Entry-lck-5.5kb

    Plasmid
    #58890
    Purpose
    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    Use
    Gateway 5' entry clone
    Mutation
    mutated single nucleotide (T-->C, 13th nucleot…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58890-plasmid-map-sequence-id-91281

    Enlarge

  5. Clover2-C1

    Plasmid
    #54711
    Purpose
    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  6. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111428-plasmid-map-sequence-id-212463

    Enlarge

  9. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    119756-plasmid-map-sequence-id-233665

    Enlarge

  12. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    119755-plasmid-map-sequence-id-233662

    Enlarge

  15. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138146-plasmid-map-sequence-id-272533

    Enlarge

Showing: 2761 - 2780 of 62913 results