We narrowed to 13,574 results for: gan

1. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

   Plasmid

   #61966

   Purpose

   Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-ME(pS)VD
   TRAP(RK)-C-mCherry
   

   Expression

   Bacterial

   Promoter

   PBAD and PLtetO

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCOLA-Gent-EM7-Erv1p

   Plasmid

   #202484

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   EM7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJOG536

   Plasmid

   #105423

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   Nuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM6-Amp-T7-roGFP

   Plasmid

   #202464

   Purpose

   Expresses redox sensitive GFP (roGFP) with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Tags

   6X-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-ERK2 K52R

   Plasmid

   #118298

   Purpose

   Expression of Rat ERK2

   Depositor

   Philip Stork

   Article

   Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

   Insert

   ERK2 K52R (Mapk1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K52R

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

   Plasmid

   #159900

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mcherry | cbr-tbb-2 UTR
   

   Expression

   Worm

   Promoter

   Pmlc-1

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJOG641

   Plasmid

   #105427

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   GAL4-VP16-GR (transcription factor: inducible expression; dexamethasone)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JH04

   Plasmid

   #73513

   Purpose

   mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mRuby2::PH::tbb-2
   

   Tags

   mRuby2

   Promoter

   mex-5

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTEI042

   Plasmid

   #105351

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   Gal4UAS & 35S min promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME18S-3HA-mULK1

    Plasmid

    #22896

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Biochem Biophys Res Commun. 1998 May 8.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

    Plasmid

    #159896

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
    

    Expression

    Worm

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CH00X

    Plasmid

    #73517

    Purpose

    TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::TagRFP-T::PH::tbb-2
    

    Tags

    TagRFP-T

    Promoter

    mes-5

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_Pro-IL-36α

    Plasmid

    #214343

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36A (IL36A Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-FKBP-empty

    Plasmid

    #221239

    Purpose

    Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-4xFKBP scaffold
    

    Use

    Retroviral

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    pGRR-r11-r10

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-mEGFP-PKCg-C1

    Plasmid

    #112266

    Purpose

    ITRACKgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME18S-3HA-mULK1(K46N)

    Plasmid

    #22897

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Biochem Biophys Res Commun. 1998 May 8.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Aspargine

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pAGM28521

    Plasmid

    #105359

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    EC1.2en-EC1.1p fusion promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-Myc-Caspase-4_W267N

    Plasmid

    #214311

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Mutation

    W267N

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pgRNA-guideless

    Plasmid

    #129464

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Guideless gRNA backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge