We narrowed to 7,351 results for: ski

1. pCellFree_G06_ZIKV NS5

   Plasmid

   #145358

   Purpose

   Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS5
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSI-check2-Tab1-3'UTR

   Plasmid

   #97155

   Purpose

   For dual luciferase report assay

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   Tab1 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS NL4-3 envFS eGFP Δnef/U3

   Plasmid

   #101346

   Purpose

   Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta nef/LTR

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. tCRMod-GGSGGS7

   Plasmid

   #111761

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS7-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. tCRMod-GGSGGS5

   Plasmid

   #111760

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS5-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ839

   Plasmid

   #39317

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (NMA0631 Neisseria meningitidis)
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

7. RSRF-Luc-2mut

   Plasmid

   #31819

   Depositor

   Astar Winoto

   Article

   Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

   Insert

   Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   two CTATATTTAG RSRF sites mutated to CGATATTTCG

   Promoter

   mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pmCellFree_KA1_ZIKVC

   Plasmid

   #145339

   Purpose

   Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Capsid
   

   Use

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   April 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

   Plasmid

   #112170

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   AAV

   Tags

   mRuby3

   Expression

   Mutation

   F102G

   Promoter

   Synapsin

   Available since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptagBFP-LacI

    Plasmid

    #103839

    Purpose

    Flourescent marker for lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-LacI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    SV2

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. miR-vector-RFP pCDNA

    Plasmid

    #97143

    Purpose

    for expression of miRNA and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1-Muc2D1D2D3

    Plasmid

    #155215

    Purpose

    Expresses human MUC2 residues 21-1259

    Depositor

    Deborah Fass

    Article

    Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

    Insert

    Muc2 D1D2D3 (MUC2 Human)
    

    Use

    Tags

    6xHistadine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCellFree_KA2_ZIKV E

    Plasmid

    #145361

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Use

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.hSynap.iGABASnFR.R205A

    Plasmid

    #112162

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R205A (no GABA binding)

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GST-Cav FL (1-178)

    Plasmid

    #22445

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

    Insert

    Caveolin1(1-178)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3'-3xFLAG minimal CMV pCDNA5

    Plasmid

    #212045

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.hSynap.iGABASnFR.F102Y.Y137L

    Plasmid

    #112161

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.GFAP.iGABASnFR.F102Y.Y137L

    Plasmid

    #112174

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    GFAP

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-51b(+) ChemoG 3.2

    Plasmid

    #193803

    Purpose

    Protein production of ChemoG3.2 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG 3.2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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