We narrowed to 9,521 results for: try

1. MAC-SARS-CoV-2 NSP8

   Plasmid

   #158359

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP8 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MAC-SARS-CoV-2 NSP10

   Plasmid

   #158361

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP10 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-CA TAG93

   Plasmid

   #105667

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 93

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-SAR1

   Plasmid

   #67344

   Purpose

   hSar1 with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. MAC-GPR78

   Plasmid

   #158382

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   GPR78 (GPR78 Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEN_TT Tgfbr3-HA

   Plasmid

   #58497

   Purpose

   Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Tgfbr3_HA (Tgfbr3 Mouse)
   

   Use

   Entry vector

   Tags

   HA

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEPQD1CB0770

   Plasmid

   #177060

   Purpose

   MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmalc4x_I499F_TEV

   Plasmid

   #47703

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with I499F mutation (MYOC Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed I499F

   Promoter

   Ptac

   Available Since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTRCHisB-TRF2_M

    Plasmid

    #50490

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 438-500

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. KP201: pMVP (L5-L4) eGFP-P2A

    Plasmid

    #121708

    Purpose

    pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL106P-LUC-ENTR1A(w451-1)

    Plasmid

    #17455

    Purpose

    Gateway entry vector, Degron N-term Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    FKBP-Luciferase fusion (MTOR firefly)
    

    Use

    Luciferase; Entry vector

    Tags

    FKBP12DD

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. 459 pME-etv2

    Plasmid

    #49002

    Purpose

    full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway middle entry

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. KP301: pMVP (L5-L4) mCherry-P2A

    Plasmid

    #121710

    Purpose

    pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPQD1CB0121

    Plasmid

    #177053

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPQD1CB0117

    Plasmid

    #177049

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid synthase (CrISY) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPQD1CB0109

    Plasmid

    #177042

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET51b-Strep-SsOGT-H5-His

    Plasmid

    #167277

    Purpose

    Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SsOGT-H5
    

    Tags

    His x10 and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HE401: pMVP (L1-R5) shRNA

    Plasmid

    #121698

    Purpose

    pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPQD1CB0108

    Plasmid

    #177041

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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