We narrowed to 407 results for: ta cloning vector

1. pLenti-nSpCas9-evoCDA1

   Plasmid

   #209042

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   evoCDA1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-opCas12a-6xNLS

   Plasmid

   #209022

   Purpose

   Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-nSpCas9-APOBEC3A

   Plasmid

   #209040

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC3A-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-enCas12a-2xNLS

   Plasmid

   #209021

   Purpose

   Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   enCas12a-2xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSR:MSMEG_0835:Dendra

   Plasmid

   #174923

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0835
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSR:MSMEG_5070:Dendra

   Plasmid

   #167375

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_5070
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSR:MSMEG_5487:Dendra

   Plasmid

   #167440

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_5487
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSR:MSMEG_5447:Dendra

   Plasmid

   #167431

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_5447
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSR:MSMEG_0310:Dendra

    Plasmid

    #159051

    Purpose

    T7 expression of C-term fluorescent fusion in E. coli

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0310
    

    Use

    Integrating mycobacterial expression vector attl5

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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