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JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

Plasmid

#156416

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G13V

Plasmid

#82800

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G13V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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KRAS-A146T

Plasmid

#154426

Purpose

A146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

Plasmid

#180468

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

KRAS4A (KRAS Human)

Use

Zebrafish transgenesis

Tags

V5-mScarlet-I

Expression

Mutation

Promoter

kdrl

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156406

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

Plasmid

#156414

Purpose

kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

mutant KRAS4B-G12V

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KRAS_p.G13C

Plasmid

#82809

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G13C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.E62K

Plasmid

#81656

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E62K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12Y

Plasmid

#82799

Purpose

Gateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Expression

Mutation

Promoter

kdrl

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

Plasmid

#156404

Purpose

Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Gateway cloning

Tags

mTagBFP2

Expression

Mutation

mutant KRAS4A-G12D

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Plasmid

#159540

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Plasmid

#159560

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Plasmid

#159562

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Plasmid

#159569

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Plasmid

#159571

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Plasmid

#159548

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R701-X55-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

Plasmid

#159563

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12V

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Plasmid

#159541

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Plasmid

#159564

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge