We narrowed to 1,924 results for: Ipp

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pCIG-FGF13A

Plasmid

#220793

Purpose

To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

Insert

FGF13 (FGF13 Human)

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-ANXA11-mCerulean

Plasmid

#164214

Purpose

pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mCerulean

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hHP1β

Plasmid

#103829

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hHP1β (CRY2, CBX1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-PGK-LAMP1-EYFP

Plasmid

#164215

Purpose

pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

EYFP

Promoter

PGK

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-blast

Plasmid

#167829

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCIBN-hTRF1-tagRFP-T

Plasmid

#103811

Purpose

BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-blast

Plasmid

#167818

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

Plasmid

#167819

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

Plasmid

#167827

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

Plasmid

#167820

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hHP1β

Plasmid

#103830

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-hHP1β (CRY2, CBX1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4 + PGK-puro

Plasmid

#167824

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV x EF1a_EKAREN4(TA)-ires-puro

Plasmid

#167830

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

Plasmid

#236241

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV1.2 (CACNA1C Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-ER WPRE

Plasmid

#236230

Purpose

AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-Sec61b WPRE

Plasmid

#236229

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Sec61b (SEC61B Human, Synthetic)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

Plasmid

#236245

Purpose

Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

Plasmid

#236240

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.2 (CACNA1B Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV hSyn INTRON mEmerald-Sec61b WPRE

Plasmid

#236228

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Sec61b (SEC61B Human, Synthetic)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-nE-mHP1alpha

Plasmid

#181895

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha-delCTE

Plasmid

#181896

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha-delCTE (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-FUSN-mHP1alpha

Plasmid

#181897

Purpose

Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

FUSN-mHP1alpha (Cbx5 Mouse, Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-PGL-1-mHP1alpha

Plasmid

#181899

Purpose

Fluorescently tagged HP1alpha fused to PGL-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGG-GFP-RGG-mHP1alpha

Plasmid

#181898

Purpose

Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

RGG-GFP-RGG (Cbx5 Mouse, Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPHR-EYFP-hGCN5mut

Plasmid

#103828

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5mut (CRY2, KAT2A Human, Mustard Weed)

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPHR-EYFP-hGCN5-NLS

Plasmid

#103827

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Human, Mustard Weed)

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge