We narrowed to 1,051 results for: SEF

1. pLD-puro-Cc-TARDBP-WT_VA

   Plasmid

   #141327

   Purpose

   Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-WT (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

   Plasmid

   #179459

   Purpose

   Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV1

   Insert

   JEDI-2P-Kv
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-human OCT4 DE-SV40-Luc

   Plasmid

   #52414

   Purpose

   Luciferase reporter plasmid for DE OCT4 enhancer activity

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   Distal element of human OCT4 promoter (POU5F1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. delta PE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52380

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted -1620 - -1011 upstream from OCT4 ATG

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. delta DE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52381

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted -3194 - -1768 upstream from OCT4 ATG

   Promoter

   OCT4

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLD-puro-Cc-TARDBP-A315T_VA

   Plasmid

   #141329

   Purpose

   Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cells

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-A315T (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

   Expression

   Mammalian

   Mutation

   A315T

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mouse deltaPE Oct4-GFP transgenic reporter

   Plasmid

   #52382

   Purpose

   BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP pA
   Oct4 (Pou5f1 Mouse)
   PGK-gb2-Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted -300 - -1300bp fragment upstream from Oct…

   Promoter

   Oct4 and PGK+gb2

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLD-puro-Cc-TARDBP-G368A_VA

   Plasmid

   #141330

   Purpose

   Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G368A in mammalian cells

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-G368A (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

   Expression

   Mammalian

   Mutation

   G368A

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLD-puro-Cc-TARDBP-G287A_VA

    Plasmid

    #141328

    Purpose

    Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G287A in mammalian cells

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-G287A (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

    Expression

    Mammalian

    Mutation

    G287A

    Promoter

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD-puro-Cc-TARDBP-W385G_VA

    Plasmid

    #141331

    Purpose

    Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-W385G in mammalian cells

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-W385G (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

    Expression

    Mammalian

    Mutation

    W385G

    Promoter

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJOE6087.3

    Plasmid

    #135082

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Insert

    P-rhaP-BAD-eGFP-His tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 2P5S

    Plasmid

    #51354

    Depositor

    Cheryl Arrowsmith

    Insert

    2P5S
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 2PMY

    Plasmid

    #51355

    Depositor

    Cheryl Arrowsmith

    Insert

    2PMY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. p2888

    Plasmid

    #198146

    Purpose

    Epitope tagging of effector protein SseG

    Depositor

    Michael Hensel

    Insert

    PsseA::sscB sseF::HA sseG::M45 (sseG Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    M45

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOpen-T7gene5

    Plasmid

    #165534

    Purpose

    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T7 DNA Polymerase (unmodified)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Rich-pT7

    Plasmid

    #108445

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MoClo adapted pUC57-Kan Level 2

    Plasmid

    #109218

    Purpose

    Non binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Rich-pT7GG

    Plasmid

    #108443

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Xenopus oocyte expression system

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MoClo adapted pUC57-Amp Level 1 position 2

    Plasmid

    #109221

    Purpose

    Non binary level 1 position 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MoClo adapted pUC57-Amp Level 1 position 6

    Plasmid

    #109225

    Purpose

    Non binary level 1 position 6 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MoClo adapted pUC57-Amp Level 1 position 4

    Plasmid

    #109223

    Purpose

    Non binary level 1 position 4 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MoClo adapted pUC57-Amp Level 1 position 1

    Plasmid

    #109220

    Purpose

    Non binary level 1 position 1 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MoClo adapted pUC57-Amp Level 1 position 5

    Plasmid

    #109224

    Purpose

    Non binary level 1 position 5 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MoClo adapted pUC57-Amp Level 1 position 7

    Plasmid

    #113044

    Purpose

    Non binary level 1 position 7 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MoClo adapted pUC57-Amp Level 1 position 3

    Plasmid

    #109222

    Purpose

    Non binary level 1 position 3 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

    Depositor

    Elena Caro Bernat

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT7TS-rich

    Plasmid

    #99050

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Unspecified; Xenopus oocyte system

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBS5

    Plasmid

    #83799

    Purpose

    Useful for tagging yeast proteins with N-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNtCp-rbcL-accD

    Plasmid

    #52469

    Purpose

    Contains rbcL and accD DNA regions for double homologous recombination useful in Nicotiana tabacum Cp transformation

    Depositor

    Anastasios Melis

    Insert

    rbcL-accd Nicotiana tabacum Cp DNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSC301

    Plasmid

    #31851

    Depositor

    Yossef Av-Gay

    Article

    Cowley et al Gene. 2001 Feb 21;264(2):225-31.

    Insert

    GFP (including ATG start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SOD

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only