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pSGE_713_Ubi-3-Trl_dCP

Plasmid

#71160

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

713_Ubi-3-Trl_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_712_Ubi-1-Trl_dCP

Plasmid

#71159

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

712_Ubi-1-Trl_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_574_S2-3-GATA_dCP

Plasmid

#71157

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

574_S2-3-GATA_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_710_S2-2-twi_dCP

Plasmid

#71156

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

710_S2-2-twi_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_475_S2-1-GATA_dCP

Plasmid

#71155

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

475_S2-1-GATA_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_530_S2-1-CGCG_dCP

Plasmid

#71154

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

530_S2-1-CGCG_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGE_646_S2-1-CACA_dCP

Plasmid

#71153

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

646_S2-1-CACA_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-NbALFA-degFusionRed

Plasmid

#199466

Purpose

Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

NbALFA-degFusionRed

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CA

Plasmid

#69616

Purpose

To express gene strongly with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Type

Empty backbone

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Use

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

HIS tag

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
pmCherry-Talin

Plasmid

#80026

Purpose

Mammalian expression of mCherry-Talin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

Talin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Smurf2 wt

Plasmid

#13504

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pRSCupSup35GFP

Plasmid

#1087

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C192A

Plasmid

#133891

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C39A

Plasmid

#133887

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

C39A

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-Vinculin

Plasmid

#80024

Purpose

Mammalian expression of mCherry-vinculin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

vinculin (VCL Chicken)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
Flag-Gsdmd-NT C265A

Plasmid

#133892

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C77A

Plasmid

#133889

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
SF-1

Plasmid

#28180

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

SF-1 (Nr5a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
Flag-Gsdmd-NT C122A

Plasmid

#133890

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C57A

Plasmid

#133888

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D09

Plasmid

#70941

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED25 (MED25 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pBlueRIP.OVA

Plasmid

#69597

Purpose

contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)

Depositor

Francis R. Carbone

Article

Blanas et al Science. 1996 Dec 6;274(5293):1707-9.

Insert

OVA (OVAL Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

rat insulin promoter (RIP)

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-S577A

Plasmid

#92235

Purpose

Express S577A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-S577A mutant

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Serine 577 to Alanine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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