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Plasmid#227294PurposeDonor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)DepositorInsertEZR Homology Arms flanking a mStayGold Tag (EZR Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2363
Plasmid#142050PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTONSL (TONSL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2046
Plasmid#144206PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARID3B (ARID3B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2045
Plasmid#144328PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARID3B (ARID3B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR218-2_nt5G>U
Plasmid#174009PurposemiR218 nt5 point mutation in SLIT3DepositorUseTagsExpressionMammalianMutationNT5 G>U point mutation in SLIT3PromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo
Plasmid#222636PurposeEzrin activation reporterDepositorInsertmiRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SNAI2_WT
Plasmid#82162PurposeGateway Donor vector containing SNAI2 , part of the Target Accelerator Plasmid Collection.DepositorInsertSNAI2 (SNAI2 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo
Plasmid#222637PurposeEzrin activation reporter, T567 phosphorylation deficientDepositorInsertmiRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)
UseLentiviralTagsmRuby3ExpressionMutationT567APromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only