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CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_pET21a

Plasmid

#27432

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q/D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pD441-H6-SUMO-HyPerFLEX

Plasmid

#216676

Purpose

Plasmid with cleavable His-tag for expression of the HyPer-FLEX sensor for hydrogen peroxide in E. coli

Depositor

Joris Messens

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis-SUMO

Expression

Bacterial

Promoter

T5

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNRCreBle

Plasmid

#234431

Purpose

Picochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal

Depositor

Matthew Posewitz

Article

Burch et al Front Microbiol. 2025 Jul 14;16:1588625.

Insert

Cre recombinase

Use

CRISPR, Cre/Lox, and Synthetic Biology ; Green al…

Mutation

Picochlorum celeri endoglucanase intron insertion

Promoter

P celeri nitrate reductase

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_010_psbD_5'Hom_Cr

Plasmid

#235788

Purpose

5' Homology psbD in the M1 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbD locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_005_rbcL_5'Hom_Cr

Plasmid

#235786

Purpose

5' Homology rbcL in the M1 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the rbcL locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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csd2_121-308/pET21a(+)

Plasmid

#83293

Purpose

csd2, construct: 121-308, pET21a(+) no-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_140-251/pET21a(+)

Plasmid

#83294

Purpose

csd2, construct:140-251, pET21a(+) C-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acids 1-139 and 252-308

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd1_54-312/pET21a(+)

Plasmid

#83291

Purpose

csd1, construct: 54-312, pET21a(+) C-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-53

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308/pET28b(+)

Plasmid

#83292

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd1_125-312/pET28b(+)

Plasmid

#83290

Purpose

csd1, construct: 125-312, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-124

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Tv9nL

Plasmid

#188069

Purpose

PROSS-designed high-redox potential laccase from Trametes versicolor

Depositor

Sarel Fleishman

Article

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

PROSS-designed high-redox potential laccase from Trametes versicolor

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

79 PROSS mutations, described in publication

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/RbcX2/BSD2/RbcX1

Plasmid

#229513

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,RbcX2,BSD2,RbcX1

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
RbcX2
BSD2
RbcX1

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDEINO1

Plasmid

#179472

Purpose

Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Inserts

ori
cat

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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J-sRNA-331Bb

Plasmid

#78285

Purpose

An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

j-sRNA-331

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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