Skip to main content
Addgene
Showing: 261 - 280 of 3414 results

  1. pT7CFE1_EVA71_VP12A

    Plasmid
    #212977
    Purpose
    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212977-plasmid-map-sequence-id-420362

    Enlarge

  2. pT7CFE1_EVD68_VP12A

    Plasmid
    #212984
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212984-plasmid-map-sequence-id-420357

    Enlarge

  3. pT7CFE1_EVD68_3ABC

    Plasmid
    #212979
    Purpose
    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212979-plasmid-map-sequence-id-420356

    Enlarge

  4. pT7CFE1_EVD68_3ABCD

    Plasmid
    #212981
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212981-plasmid-map-sequence-id-420354

    Enlarge

  6. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid
    #52053
    Purpose
    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
    Depositor
    Jesse Rinehart
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetR/pLtetO
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52053-plasmid-map-sequence-id-442992

    Enlarge

  7. pT7CFE1_polio_3ABC

    Plasmid
    #212986
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212986-plasmid-map-sequence-id-420355

    Enlarge

  12. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

    Enlarge

Showing: 261 - 280 of 3414 results