We narrowed to 2,316 results for: pET28

1. pET28a-cMSPdH45

   Plasmid

   #199767

   Purpose

   Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH45
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)-SUMO-A'-l4-G'

   Plasmid

   #209124

   Purpose

   Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Tyr-P(11)4

   Plasmid

   #185784

   Purpose

   Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   Tyr-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a(CTLA4)

   Plasmid

   #177905

   Purpose

   Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CTLA4 (aa 183-223 only) (CTLA4 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a(DAP10)

   Plasmid

   #177904

   Purpose

   Bacterial expression of his10-tagged human DAP10 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   DAP10 (aa 70-92 only) (HCST Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-FAM86A

   Plasmid

   #85117

   Purpose

   bacterial expression of FAM86A

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EEF2KMT (FAM86A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Vx3(A7)

   Plasmid

   #35526

   Depositor

   Robert Cohen

   Article

   Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

   Insert

   Vx3(A7)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Changed G2 to P

   Promoter

   T7

   Available since

   April 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-MSP1D1deltaH4/2

   Plasmid

   #71712

   Purpose

   Membrane scaffold protein (MSP), half of helix 4 truncated

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-SznE

   Plasmid

   #139282

   Purpose

   expresses SznE in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   sznE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-soNadV

    Plasmid

    #83363

    Purpose

    Expresses nadV from Shewanella oneidensis in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Insert

    nadV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-mTDGa.1

    Plasmid

    #81051

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28b-pvdA WT

    Plasmid

    #87098

    Purpose

    expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

    Insert

    pvdA WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-PAN

    Plasmid

    #200971

    Purpose

    Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

    Depositor

    Yifan Cheng

    Article

    Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

    Insert

    PAN
    

    Use

    Tags

    6xHis and 6xHis-thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-TmCA-P(11)4

    Plasmid

    #185783

    Purpose

    Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    TmCA-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a MDH_PFALCI

    Plasmid

    #204235

    Purpose

    Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28b-NpR5600

    Plasmid

    #45741

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    NpR5600 (Npun_R5600 Nostoc punctiforme)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-nGFPMNase

    Plasmid

    #187826

    Purpose

    express nGFPMNase protein in E coli

    Depositor

    Yikang Rong

    Article

    Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

    Insert

    nGFPMNASE
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promotor

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28b-PU.1-ETS

    Plasmid

    #85734

    Purpose

    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

    Depositor

    Gregory Poon

    Article

    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

    Insert

    ETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
    

    Use

    Tags

    6xHis following thrombin cleavage site LVPR|G

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge