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pQO18-TEV

Plasmid

#27399

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX10 and TEV

Expression

Bacterial

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pETO18G-AP

Plasmid

#27402

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

Biotin Acceptor Domain (BAD), HisX8, and Thrombin…

Expression

Bacterial

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pQO18 TEV-L400C

Plasmid

#27400

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX10 and TEV

Expression

Bacterial

Mutation

Changed Leu 400 to Cys

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pKMW123_SpRY-Cas9_U6-entry

Plasmid

#244820

Purpose

Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSM019

Plasmid

#128291

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-sfyfp-parB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJJB1402

Plasmid

#218616

Purpose

Express Pex15, Pex22, Pex4, Pex1, and Pex6

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex15

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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GST-Ub4

Plasmid

#171421

Purpose

For the purifcation of tetra linear ubiquitin

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

Ubiquitin

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCC28

Plasmid

#128284

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, sopC

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1148

Plasmid

#119029

Purpose

Yeast expression plasmid for PmCYP719A26

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmCYP719A26

Expression

Yeast

Promoter

GAL10

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJJB1578

Plasmid

#218620

Purpose

Express Pex22, Pex6, Pex15, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAA3

Plasmid

#128280

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSM040

Plasmid

#128294

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, parS

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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NaChBac pTracer CMV2

Plasmid

#60835

Purpose

Mammalian expression plasmid containing NaChBac gene with an EGFP reporter

Depositor

David Clapham

Article

Ren et al Science. 2001 Dec 14;294(5550):2372-5.

Insert

NaChBac

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pJKW0770

Plasmid

#119023

Purpose

E.coli expression plasmid for PmSPS1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW0957

Plasmid

#119026

Purpose

E.coli expression plasmid for PmKOMT1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmKOMT1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW0776

Plasmid

#119024

Purpose

E.coli expression plasmid for PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS2

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSM015

Plasmid

#128289

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-cfp-parB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAA6

Plasmid

#128282

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq- mRFP, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGMCZq17-OXOX

Plasmid

#31289

Depositor

Dirk Schnappinger

Article

Venugopal et al Cell Host Microbe. 2011 Jan 20. 9(1):21-

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only