We narrowed to 2,459 results for: GEM

1. KAT6B-luciferase

   Plasmid

   #113353

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   KAT6B-enhancer (KAT6B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2R3a-3xVenusYFP-OcsT

   Plasmid

   #71271

   Purpose

   Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   3 times VenusYFP
   

   Use

   Gateway

   Tags

   octaline synthase terminator

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB N-term-mNeongreen

   Plasmid

   #169226

   Purpose

   Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

   Plasmid

   #66779

   Purpose

   The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

   Depositor

   Thomas Oertner

   Article

   Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

   Inserts

   hbeRhGC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn-somAion-citrine

   Plasmid

   #192570

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   somAion
   

   Use

   AAV

   Tags

   Citrine - soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2R3a-GUS-OcsT

   Plasmid

   #71267

   Purpose

   Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Tags

   2xGly linker and octaline synthase terminator

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MKV937/nlp-1p::FlincG3

   Plasmid

   #140508

   Purpose

   This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

   Depositor

   Miri VanHoven

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   nlp-1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

   Plasmid

   #113953

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S, S212N, and …

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMACE DEST CMV SpCas9

   Plasmid

   #206268

   Purpose

   DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-X1-Neo-GFP-ATL1-G20TM

    Plasmid

    #109019

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-deltaTM (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    truncated form without C-terminal ER transmembran…

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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