We narrowed to 3,005 results for: EED

1. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133345

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3a_023

   Plasmid

   #123775

   Purpose

   Encodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   his::ABI::tagBFP::linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTCW015

   Plasmid

   #83530

   Purpose

   UBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoter

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Insert

   pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pFUS1J2

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

   Plasmid

   #133341

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ220-Ultra

   Plasmid

   #176889

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   AsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ230-E795L

    Plasmid

    #176890

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    LbCas12a-E795L
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLV3Cas-LT1

    Plasmid

    #210549

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    2bp insertion after AAVS1 target sequence that di…

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Myc-XPLN

    Plasmid

    #73365

    Purpose

    Expresses myc tagged XPLN in mammalian cells

    Depositor

    Krister Wennerberg, Jie Chen

    Article

    Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Use

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pPBT-PE2-PGK-Blast

    Plasmid

    #173219

    Purpose

    piggyBac transposon containing PE2 and Blasticidin expression cassettes

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    Blast
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and hPGK

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p CIneo-RL-Let7-3xBulgeB-mut

    Plasmid

    #115369

    Purpose

    Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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