We narrowed to 2,459 results for: GEM

1. AAV2_hSyn_Phobos_Citrine

   Plasmid

   #98216

   Purpose

   Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

   Promoter

   human synapsin

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS04 [myo-3p::bPGC::SL2::mCherry]

   Plasmid

   #168172

   Purpose

   Expression of bPGC in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   bPGC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB C-term-Dendra2

   Plasmid

   #169216

   Purpose

   Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked Dendra2-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFrt-invCAG-Luc

   Plasmid

   #63577

   Purpose

   Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites

   Depositor

   Ivo Huijbers

   Article

   Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

   Insert

   Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
   

   Use

   Cre/Lox; Frt/flpe recombination mediated insertion

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-DDRGK1-K-less-HA

   Plasmid

   #139849

   Purpose

   Lentiviral expression of human DDRGK1-K-less-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   K116R, K120R, K121R,K124R, K128R, K146R, K176R, K…

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CMV-GFP-Puro_TAL1-long

   Plasmid

   #210642

   Purpose

   Overexpression of TAL1-long

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-long (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-GFP-Puro_TAL1-short

   Plasmid

   #210641

   Purpose

   Overexpression of TAL1-short

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-short (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-pLKO-puro-shTAL1

   Plasmid

   #210640

   Purpose

   Knockdown of TAL1 endogenous (3'UTR)

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1 3' UTR gRNA (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMK ENTR 1 HITI-2c ACTB

   Plasmid

   #206269

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   HITI-2c donor
   

   Use

   CRISPR; Multimate/gateway entr 1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid

    #182682

    Purpose

    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NA

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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