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We narrowed to 13,007 results for: cdna

Showing: 5601 - 5640 of 13007 results

  1. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  2. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid
    #138212
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138212-plasmid-map-sequence-id-307414

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  15. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid
    #65727
    Purpose
    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.
    Depositor
    Jacco van Rheenen
    Article
    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1
    Inserts
    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and UBC
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    65727-plasmid-map-sequence-id-243566

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  26. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  35. pcDNA3-mNG-IRES-mScarlet-dMCP

    Plasmid
    #231089
    Purpose
    Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.
    Depositor
    John Ngo
    Insert
    mNG-IRES-mScarlet-dMCP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231089-plasmid-map-sequence-id-458496

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  37. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid
    #162280
    Purpose
    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN8A Variant 1, stabilized (SCN8A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    Modified human HSPA5 intron 4 inserted after bp23…
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    162280-plasmid-map-sequence-id-334621

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  40. pcDNA3 mCherry LIC cloning vector (6B)

    Plasmid
    #30125
    Purpose
    Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    30125-plasmid-map-sequence-id-174010

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Showing: 5601 - 5640 of 13007 results