...region of a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted...to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and...to choose an appropriate promoter to express your cDNA, depending on the level of expression you would ...

...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate...

... set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them into lentiviral...months from start to finish. They cloned all the cDNAs into a single consensus lentiviral expression vector...

...simpler to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual...highlight a key advantage of CRISPRa over traditional cDNA overexpression: isoform-specific gene expression...— at least, more straightforward than generating cDNA for each gene isoform individually. To highlight...application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still be preferable...strategies) and then consider whether traditional cDNA overexpression is suited to your experimental goals...

...large scale, broadly available collection of mutant cDNA clones corresponding to cancer variants found in... genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular pathways...Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived ...

...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product ...

...they want to activate, we would recommend using a cDNA overexpression vector rather than going through ...protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express...and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis regulation and also...

...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...

...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by...

...purposes of this tutorial we will discuss how to move a cDNA from one plasmid to another. However, the same technique... problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...

...expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression...EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW ...FUGW 3rd hUbC-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and...plasmid with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use a wide variety of drug...3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic...3rd Gateway plasmid for CMV driven expression of cDNA. See article for alternative versions of this plasmid...21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for...

..., reverse transcription of RNA such as long-read cDNA sequencing and RNA sequencing of complex heterogeneous...

... from the publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq. This...

...myeloma cell. This RNA was then used to generate cDNA, which  was used as a template for PCR amplification...

...expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on...

...to certain gene sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries...PCR-amplified and sequenced using NGS to identify their cDNA or target gene. Positive selection screens are generally...

...In this approach, viral RNA is first converted to cDNA and then quantified using qPCR primers targeting...

...adaptable to cloning different types of DNA, such as cDNA, PCR fragments, or genomic DNA. Also remember that...

...over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene...

...sybody candidates are then reverse transcribed into cDNA fragments that are efficiently subcloned into the...