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  1. Guide to Using Pooled Libraries

    Type
    Guide
    ...to certain gene sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries...PCR-amplified and sequenced using NGS to identify their cDNA or target gene. Positive selection screens are generally...
  2. Lentiviral Guide

    Type
    Guide
    ... simple transfections. Is it feasible to express cDNA from a lentiviral transfer plasmids normally used...drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus requires...
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